Reporter

Part:BBa_K4205001:Design

Designed by: Xinyuan Wang   Group: iGEM22_NJMU-China   (2022-09-04)


PpbrA-PmerR+Lpp-OmpA-MT1A


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 58
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 58
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 58
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 584


Design Notes

When assembling BBa_K4205002 and BBa_K4205000, we added a SCAR sequence（TACTAG） between them to prevent spatial effects.


Source

The composite parts are assembled from BBa_K4205002 and BBa_K4205000.

References